Identification and evaluation of reference genes in the Chinese white wax scale insect Ericerus pela

BACKGROUND The Chinese white wax scale insect, Ericerus pela, is a well-known resource insect. The females and males are dramatically distinct at each developmental stage. We sought to identify suitable reference genes to use as internal controls in molecular research on E. plea. RESULTS geNorm, RefFinder and Normfinder analyses showed that ßTub-2 was the best reference gene throughout different developmental stages; SdhA-1 was the most stable reference gene in different tissues, and ßTub-1 was the most reliable reference gene under treatment with different temperatures. The results also showed that the optimal number of reference genes for analyzing target gene expression levels in the three experimental conditions was two. CONCLUSIONS The identified reference genes are suitable reference genes for normalization in RT-qPCR of E. pela samples.